rabbit polyclonal anti cb 2  (Alomone Labs)

Journal: PLoS ONE

Article Title: Reduced Endothelium-Dependent Relaxation to Anandamide in Mesenteric Arteries from Young Obese Zucker Rats

doi: 10.1371/journal.pone.0063449

Figure Lengend Snippet: Cumulative concentration-response curves to anandamide ( A ), ACEA, CB 1 receptor-selective agonist ( B ), JWH-015, CB 2 receptor-selective agonist ( C ), capsaicin, vanilloid receptor agonist ( D ), acetylcholine (ACh), endothelium-dependent vasodilator ( E ) and sodium nitroprusside (SNP), endothelium-independent vasodilator ( F ) in U46619-precontracted mesenteric arteries from lean Zucker rats (LZRs) and obese Zucker rats (OZRs). Each point represents the mean ± SEM. *, P<0.05 vs. LZR. N = 6/group.

Article Snippet: The sections were subsequently incubated (at the indicated dilutions) with rabbit monoclonal anti-CB 1 (1∶100, Abcam), rabbit polyclonal anti-CB 2 (1∶100, Abcam), or mouse anti-von Willebrand (1∶500, Abcam) overnight at 4°C.

Techniques: Concentration Assay

Journal: PLoS ONE

Article Title: Reduced Endothelium-Dependent Relaxation to Anandamide in Mesenteric Arteries from Young Obese Zucker Rats

doi: 10.1371/journal.pone.0063449

Figure Lengend Snippet: In A , the vessels were pretreated with a CB 1 antagonist (AM 251, 1 µM) and in B with a CB 2 antagonist (AM 630, 1 µM) for 30 minutes. The relaxation to anandamide was evaluated in U46619-precontracted mesenteric arteries from lean Zucker rats (LZRs) and obese Zucker rats (OZRs). Each point represents the mean ± SEM. *, P<0.05 vs. LZR. #, P<0.05 vs. respective group in the absence of blockade. N = 6/group.

Article Snippet: The sections were subsequently incubated (at the indicated dilutions) with rabbit monoclonal anti-CB 1 (1∶100, Abcam), rabbit polyclonal anti-CB 2 (1∶100, Abcam), or mouse anti-von Willebrand (1∶500, Abcam) overnight at 4°C.

Techniques:

Journal: PLoS ONE

Article Title: Reduced Endothelium-Dependent Relaxation to Anandamide in Mesenteric Arteries from Young Obese Zucker Rats

doi: 10.1371/journal.pone.0063449

Figure Lengend Snippet: Panels show densitometric analysis of the Western blots for CB 1 and CB 2 protein expression in vessels from lean Zucker rats (LZRs) and obese Zucker rats (OZRs). In A and B , Western blots for CB 1 and CB 2 receptors, respectively. In C , Western blots for TRPV1 receptors. Results were normalized to β-actin expression and expressed as units of change from the control. Data are expressed as mean ± SEM. *, P<0.05 vs. LZR. N = 5/group.

Article Snippet: The sections were subsequently incubated (at the indicated dilutions) with rabbit monoclonal anti-CB 1 (1∶100, Abcam), rabbit polyclonal anti-CB 2 (1∶100, Abcam), or mouse anti-von Willebrand (1∶500, Abcam) overnight at 4°C.

Techniques: Western Blot, Expressing

Journal: PLoS ONE

Article Title: Reduced Endothelium-Dependent Relaxation to Anandamide in Mesenteric Arteries from Young Obese Zucker Rats

doi: 10.1371/journal.pone.0063449

Figure Lengend Snippet: Panels show confocal microscopic images of von Willebrand factor (vWF, red), DAPI (blue), CB 1 and CB 2 receptors (green) in mesenteric arteries from lean Zucker rats (LZRs, A and B ) and obese Zucker rats (OZRs, C and D ). Endothelium-intact mesenteric sections were immunolabeled with antibodies against CB 1 , CB 2 and von Willebrand factor. The nuclei were counterstained with DAPI. An overlay of the vWF, DAPI and CB 1 or CB 2 images is presented (merge). Endothelial localization of CB 1 and CB 2 receptors is shown in the merged images (yellow). The images are representative of three separated experiments. N = 5/group. Scale bar = 40 µm.

Article Snippet: The sections were subsequently incubated (at the indicated dilutions) with rabbit monoclonal anti-CB 1 (1∶100, Abcam), rabbit polyclonal anti-CB 2 (1∶100, Abcam), or mouse anti-von Willebrand (1∶500, Abcam) overnight at 4°C.

Techniques: Immunolabeling

Journal: Nanomaterials

Article Title: ZnO Nanoparticles Induce Dyslipidemia and Atherosclerotic Lesions Leading to Changes in Vascular Contractility and Cannabinoid Receptors Expression as Well as Increased Blood Pressure

doi: 10.3390/nano11092319

Figure Lengend Snippet: Effect of ZnONPs on aorta contractility. ( a ) Effect of ACPA (CB 1 receptor agonist) on contraction in aortic rings in different experimental groups. ( b ) Effect of HU308 (CB 2 receptor agonist) on contraction in aortic rings in different experimental groups. Blue bars correspond to the control non-treated group, green bars correspond to the ZnONPs treated-groups at different times; ( a ) there is a significant difference compared to the phenylephrine group with p < 0.05; ( b ) there is a significant difference compared to the control group with p < 0.05.

Article Snippet: Next, aorta sections were blocked (Animal-free blocker and diluent, Vector laboratories Inc., Burlingame, CA, USA) and incubated with rabbit polyclonal Anti-CB 1 (1:100, Alomone, Labs, Jerusalem, Israel) or rabbit polyclonal Anti-CB 2 (1:100, Alomone, Labs, Jerusalem, Israel) primary antibody and co-labeled with mouse monoclonal Anti-alpha smooth muscle Actin antibody (1:200, Abcam, Cambrige, UK) overnight.

Techniques:

Journal: Nanomaterials

Article Title: ZnO Nanoparticles Induce Dyslipidemia and Atherosclerotic Lesions Leading to Changes in Vascular Contractility and Cannabinoid Receptors Expression as Well as Increased Blood Pressure

doi: 10.3390/nano11092319

Figure Lengend Snippet: Effect of ZnONPs on the CB 1 and CB 2 receptors expression in the aorta wall. ( a ) CB 1 expression, ( b ) CB 2 expression. Blue bars correspond to the control non-treated group, green bars correspond to the ZnONPs treated-groups at different times; ( a ) there is a significant difference compared to the control group with p < 0.05.

Article Snippet: Next, aorta sections were blocked (Animal-free blocker and diluent, Vector laboratories Inc., Burlingame, CA, USA) and incubated with rabbit polyclonal Anti-CB 1 (1:100, Alomone, Labs, Jerusalem, Israel) or rabbit polyclonal Anti-CB 2 (1:100, Alomone, Labs, Jerusalem, Israel) primary antibody and co-labeled with mouse monoclonal Anti-alpha smooth muscle Actin antibody (1:200, Abcam, Cambrige, UK) overnight.

Techniques: Expressing

Journal: Nanomaterials

Article Title: ZnO Nanoparticles Induce Dyslipidemia and Atherosclerotic Lesions Leading to Changes in Vascular Contractility and Cannabinoid Receptors Expression as Well as Increased Blood Pressure

doi: 10.3390/nano11092319

Figure Lengend Snippet: Representative images showed ZnONPs effect on CB 1 and CB 2 receptors expression in aorta wall. Transmitted light images (grey). Scale bar corresponds to 50 μm. Smooth muscle α-actin was detected with a specific antibody labeled with Alexa Fluor 568 (red). CB 1 and CB 2 located on the aorta ring were detected by specific antibodies and labeled with FITC (green). Image overlap indicates a high degree of colocalization of CB 1 or CB 2, and smooth muscle α-actin (yellow). Nuclei were counterstained with DAPI dye (blue).

Article Snippet: Next, aorta sections were blocked (Animal-free blocker and diluent, Vector laboratories Inc., Burlingame, CA, USA) and incubated with rabbit polyclonal Anti-CB 1 (1:100, Alomone, Labs, Jerusalem, Israel) or rabbit polyclonal Anti-CB 2 (1:100, Alomone, Labs, Jerusalem, Israel) primary antibody and co-labeled with mouse monoclonal Anti-alpha smooth muscle Actin antibody (1:200, Abcam, Cambrige, UK) overnight.

Techniques: Expressing, Labeling